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CRP was measured with a particle-enhanced- immunonephelometric assay and a Dade Behring BN11 nephelometer (Dade Behring, Inc., Deerfield, IL). Intraassay and interassay CVs ranged from 2.3 to 4.4% and from 2.1 to 5.7%, respectively.Smoking history was determined from self-report and dichotomized in the analysis as “current smoking” “ever smoked” or “never smoked.” Education was assessed as years of schooling.Using data from the In CHIANTI Study, we hypothesized a negative relationship between SHBG and a positive relationship between sex hormones and inflammatory markers in late postmenopausal women.
It has been suggested that the mild proinflammatory state that is often detected in older persons is connected with the hormonal milieu occurring with aging (2).
In women, the abrupt decline in circulating estradiol (E2) levels during menopause together with the smaller decline in testosterone levels leads to a dramatic increase in testosterone to estrogen ratio (4).
For TNF-α, intraassay CVs ranged from 1.4 to 7.9%, whereas the interassay CV was less than 21%; for s IL-6r, intraassay and interassay CVs were less than 6.0% and less than 10.0%, respectively.
For IL-6, the interassay and intraassay CVs were 7%.
Physical activity in the year before the interview was coded as: 1) sedentary, completely inactive or light-intensity activity less than 1 h/wk; 2) light physical activity, light-intensity activity 2–4 h/wk; and 3) moderate to high physical activity, light activity at least 5 h/wk or more or moderate activity at least 1–2 h/wk.
Weight and height were measured using standard techniques.
In adult women, SHBG levels seem to mirror insulin and body mass index (BMI) trajectories (15), supporting its role as an independent predictor of metabolic syndrome and type 2 diabetes (16–18) conditions, both characterized by low-grade inflammatory status (19).
Although several lines of research suggest a possible relationship between sex hormones and a proinflammatory state, especially in postmenopausal women, such a relationship has been only partially explained in large populations and with conflicting findings (9, 20–25).
The MDC was 0.08 nmol/liter; intraassay and interassay CVs for three different concentrations were 9.6, 8.1, and 7.8%, and 8.6, 9.1, and 8.4%, respectively.
SHBG was measured using immunoradiometric assay (Diagnostic Products, Los Angeles, CA) with an MDC of 3.00 nmol/liter, and inter- and intraassay CV concentrations for three different concentrations were 3.7, 1.1, and 3.4% and 11.5, 10.3, and 8.7%, respectively.
Blood samples were obtained from participants after a 12-h fast and after a 15-min rest.